Ineffective siRNAs              
ID gene name accession number start codon position termi-
nating codon position
target site position length of mRNA target site length of siRNA
1 human Tissue Factor (hTF) M16553.1 76 963 75 2104 CATGGAGACCCCTGCCTGGCCCC 21
2 human Tissue Factor (hTF) M16553.1 76 963 156 2104 CCAGGTGGCCGGCGCTTCAGGCA 21
3 human Tissue Factor (hTF) M16553.1 76 963 159 2104 GGTGGCCGGCGCTTCAGGCACTA 21
4 human Tissue Factor (hTF) M16553.1 76 963 162 2104 GGCCGGCGCTTCAGGCACTACAA 21
5 human Tissue Factor (hTF) M16553.1 76 963 168 2104 CGCTTCAGGCACTACAAATACTG 21
6 human Tissue Factor (hTF) M16553.1 76 963 171 2104 TTCAGGCACTACAAATACTGTGG 21
7 human Tissue Factor (hTF) M16553.1 76 963 174 2104 AGGCACTACAAATACTGTGGCAG 21
8 human Tissue Factor (hTF) M16553.1 76 963 254 2104 AACCCGTCAATCAAGTCTACACT 21
9 human Tissue Factor (hTF) M16553.1 76 963 457 2104 AACTCCCCAGAGTTCACACCTTA 21
10 human Tissue Factor (hTF) M16553.1 76 963 476 2104 CTTACCTGGAGACAAACCTCGGA 21
11 human Tissue Factor (hTF) M16553.1 76 963 560 2104 AACGGACTTTAGTCAGAAGGAAC 21
12 human Tissue Factor (hTF) M16553.1 76 963 927 2104 GAGCTGGAAGGAGAACTCCCCAC 21
13 human protein serine kinase H1 (PSKH1) AJ272212.1 131 1405 312 3460 CCTGCCCCGGTCCCCCGACTGCT 21
14 human protein serine kinase H1 (PSKH1) AJ272212.1 131 1405 544 3460 GGTGTGTGAGTCGGAGCTGCGTG 21
15 human protein serine kinase H1 (PSKH1) AJ272212.1 131 1405 564 3460 GTGTGCTGCGTCGGGTGCGTCAT 21
16 human protein serine kinase H1 (PSKH1) AJ272212.1 131 1405 737 3460 CTGGATGGCGTCCGGTATCTGCA 21
17 human protein serine kinase H1 (PSKH1) AJ272212.1 131 1405 130 3460 GATGGGCTGTGGGACAAGCAAGG 21
18 human protein serine kinase H1 (PSKH1) AJ272212.1 131 1405 1382 3460 TACCAGCAGCAATACAATGGCTG 21
  Remarks: All ineffective siRNAs are from the paper: Holen T, Amarzguioui M, Wiige, MT, Babaie E, and Prydz H. (2002). Positional effects of short interfering RNAs targeting the human coagulation trigger tissue factor. Nucl. Acids Res. 30(8):1757-1766